![]() The vaginal microbiome has the potential to influence the conditions in the uterine cavity where implantation occurs in the uterine luminal epithelium, since vaginal bacteria can ascend into the uterine cavity. Similar findings are supported by others, in women and in the male. , Escherichia coli and/or Streptococcus sp. įurther, women who harboured anaerobes notably Gardnerella vaginalis causing bacterial/anaerobic vaginosis and potential pathogens Enterococcus sp. The major findings were that women whose vagina was dominated by a variety of Lactobacillus species had the most favourable pregnancy outcome and is still considered an indicator of vaginal health by maintaining an acidic pH to aid protection from the effect of anaerobes and Escherichia coli. ![]() Researching the urogenital microbiome using next-generation sequencing (NGS) of the 16S ribosomal RNA gene has been reported. Implantation rates worldwide are still disappointingly low and few reliable predictive tests are available. Lack of implantation of an embryo into the endometrium in women undergoing IVF is far too common an occurrence. ![]() Using a rapid antigen test, a woman can self-sample prior to embryo transfer and obtain an indication of microbial species present which could influence implantation outcome. Using the indicators detected to have a significant influence, these results can be extrapolated. This methodology is most suitable as a foundation on which to develop a timely test of microbiome profiling. This methodology has a substantial advantage of being affordable and easily performed in any routine molecular laboratory. The addition of further microbial targets (yet to be determined) could be combined in this predictive test for vaginal preparedness on the day of embryo transfer. The results provide evidence that most other microbial species chosen for testing had little functional effect on implantation rates. Samples from women at the time of Embryo Transfer who did not achieve implantation had significantly higher percent of samples that were positive for Prevotella bivia and Staphylococcus aureus compared to women who did achieve implantation. The qPCR result was interpreted qualitatively using the Z proportionality test. We found that some microbial species affected implantation. We tested couples attending Fertility Associates, Christchurch, New Zealand for their first round of IVF. The test panel included a range of potential urogenital pathogens, STIs, ‘favourable bacteria’ ( Lactobacillus spp.) and ‘unfavourable bacteria’ (anaerobes) reported to influence implantation rates. ![]() Using unique custom qPCRs, we tested for the presence of specific microbial species from vaginal samples and First Catch Urines from the male. This pilot study aimed to develop a methodology characterising the urogenital microbiome as a predictive test in the IVF workup. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |